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IN VITRO TEST

Cytotoxicity test

Cytotoxicity test

Content

Cytotoxicity analysis

Period

3 weeks

Figure/Image

Graph

Anti-wrinkle efficacy test

Anti-wrinkle efficacy test

Content

Skin wrinkle related gene expression analysis

Period

4 weeks

Figure/Image

Graph
Anti-wrinkle efficacy test

Content

Collagen1 protein expression analysis

Period

4 weeks

Figure/Image

Graph
Anti-wrinkle efficacy test

Content

Procollagen protein expression analysis

Period

4 weeks

Figure/Image

Graph
Anti-wrinkle efficacy test

Content

MMP1 (collagenase) protein expression analysis

Period

4 weeks

Figure/Image

Graph
Anti-wrinkle efficacy test

Content

Elastase inhibition analysis

Period

4 weeks

Figure/Image

Graph
Anti-wrinkle efficacy test

Content

Aging model design by light (UV or bluelight)-Ex vivo

Period

4 weeks

Figure/Image

Negative Control Group Negative Control Group
UVB-irradiated Group UVB-irradiated Group
Test Group Test Group

Whitening efficacy test

Whitening efficacy test

Content

Skin whitening related gene expression analysis

Period

4 weeks

Figure/Image

Graph
Whitening efficacy test

Content

Melanin production analysis

Period

4 weeks

Figure/Image

Graph
Whitening efficacy test

Content

L-DOPA oxidation inhibition analysis

Period

4 weeks

Figure/Image

Graph
Whitening efficacy test

Content

Tyrosinase inhibition analysis

Period

4 weeks

Figure/Image

Graph

Anti-oxidant efficay test

Anti-oxidant efficay test

Content

DPPH radical scavenging activity analysis

Period

2 weeks

Figure/Image

Graph
Anti-oxidant efficay test

Content

ORAC antioxidant value analysis

Period

2 weeks

Figure/Image

Graph
Anti-oxidant efficay test

Content

ROS analysis

Period

4 weeks

Figure/Image

Graph
Anti-oxidant efficay test

Content

Antioxidant enzyme analysis in skin corneous

Period

6 weeks

Figure/Image

Graph

Anti-diabetic efficacy test

Anti-diabetic efficacy test

Content

α-glucosidase inhibition analysis

Period

4 weeks

Figure/Image

Graph

Anti-glycosylation efficacty test

Anti-glycosylation efficacty test

Content

Advanced glycation end products (AGEs) analysis

Period

4 weeks

Figure/Image

Graph
Anti-glycosylation efficacty test

Content

Denatured collagen production analysis

Period

4 weeks

Figure/Image

Image

Anti-obesity efficacy test

Anti-obesity efficacy test

Content

Adipogenesis related gene expression analysis

Period

8 weeks

Figure/Image

Graph
Anti-obesity efficacy test

Content

Adipocytes lipid uptake analysis

Period

8 weeks

Figure/Image

Negative Control Group Negative Control Group
Adipogenesis-induced Group Adipogenesis-induced Group
Test Group Test Group

Moisturizing efficacy test

Moisturizing efficacy test

Content

Skin moisturizing related gene expression analysis

Period

4 weeks

Figure/Image

Graph
Moisturizing efficacy test

Content

Skin barrier related gene expression analysis

Period

4 weeks

Figure/Image

Graph

Wound healing efficacy test

Wound healing efficacy test

Content

Scratch wound healing assay

Period

4 weeks

Figure/Image

Negative Control Group Negative Control Group
Test Group Test Group

Anti-inflammation efficacy test

Anti-inflammation efficacy test

Content

Inflammation model design-Ex vivo

Period

12 weeks

Figure/Image

Graph
Anti-inflammation efficacy test

Content

Anti-inflammation related gene expression analysis

Period

4 weeks

Figure/Image

Graph
Anti-inflammation efficacy test

Content

B cell- IgE concentration analysis

Period

6 weeks

Figure/Image

Graph
Anti-inflammation efficacy test

Content

Mast cell - Histamin or IL-6 analysis

Period

6 weeks

Figure/Image

Graph
Anti-inflammation efficacy test

Content

Macrophage - TNFα or IL-6 analysis

Period

6 weeks

Figure/Image

Graph
Anti-inflammation efficacy test

Content

Keratinocyte - TARC analysis

Period

6 weeks

Figure/Image

Graph

Microdust efficacy test

Microdust efficacy test

Content

Inflammation or skin barrier related gene expression analysis

Period

8 weeks

Figure/Image

Graph
Microdust efficacy test

Content

Anti-inflammation related gene expression analysis

Period

8 weeks

Figure/Image

Graph
Microdust efficacy test

Content

ROS analysis

Period

8 weeks

Figure/Image

Image

Skin absorption efficacy test

Skin absorption efficacy test

Content

Absorption analysis in skin model

Period

6 weeks

Figure/Image

Negative Control Group Negative Control Group
Test Group Test Group

Alternatives to the eye irritation test

Alternatives to the eye irritation test

Content

Retinal cell viability analysis

Period

4 weeks

Figure/Image

Graph

Anti-hair loss efficacy test

Anti-hair loss efficacy test

Content

Human follicle dermal papilla cell proliferation analysis

Period

3 weeks

Figure/Image

Graph
Anti-hair loss efficacy test

Content

Hair loss related gene expression analysis

Period

4 weeks

Figure/Image

Graph

Hair improvement efficay test

Hair improvement efficay test

Content

InfIltration analysis of fluorescent protein to hair

Period

2 weeks/4 weeks

Figure/Image

Image
Hair improvement efficay test

Content

Hair roughness analysis

Period

4 weeks

Figure/Image

Control Group Control Group
Test Group Test Group

Anti-microbial activity in vitro

Anti-microbial activity in vitro

Content

Escherichia coli/Staphylococcus aureus/Candida albicans

Period

3 weeks

Figure/Image

Image

Anti-microbial efficacy test

Anti-microbial efficacy test

Content

Escherichia coli/Staphylococcus aureus/Candida albicans

Period

3 weeks

Figure/Image

Image
Test Content Period Figure/Image

Cytotoxicity test

Cytotoxicity analysis 3 weeks Graph

Anti-wrinkle efficacy test

Skin wrinkle related gene expression analysis 4 weeks Graph
Collagen1 protein expression analysis 4 weeks Graph
Procollagen protein expression analysis 4 weeks Graph
MMP1 (collagenase) protein expression analysis 4 weeks Graph
Elastase inhibition analysis 4 weeks Graph
Aging model design by light (UV or bluelight)-Ex vivo 6 weeks
Images View
Negative Control Group Negative Control Group
UVB-irradiated Group UVB-irradiated Group
Test Group Test Group

Whitening efficacy test

Skin whitening related gene expression analysis 4 weeks Graph
Melanin production analysis 4 weeks Graph
L-DOPA oxidation inhibition analysis 4 weeks Graph
Tyrosinase inhibition analysis 4 weeks Graph

Anti-oxidant efficay test

DPPH radical scavenging activity analysis 2 weeks Graph
ORAC antioxidant value analysis 2 weeks Graph
ROS analysis 4 weeks Graph
Antioxidant enzyme analysis in skin corneous 6 weeks Graph

Anti-diabetic efficacy test

α-glucosidase inhibition analysis 4 weeks Graph

Anti-glycosylation efficacty test

Advanced glycation end products (AGEs) analysis 4 weeks Graph
Denatured collagen production analysis 4 weeks Image

Anti-obesity efficacy test

Adipogenesis related gene expression analysis 8 weeks Graph
Adipocytes lipid uptake analysis 8 weeks
Images View
Negative Control Group Negative Control Group
Adipogenesis-induced Group Adipogenesis-induced Group
Test Group Test Group

Moisturizing efficacy test

Skin moisturizing related gene expression analysis 4 weeks Graph
Skin barrier related gene expression analysis 4 weeks Graph

Wound healing efficacy test

Scratch wound healing assay 4 weeks
Images View
Negative Control Group Negative Control Group
Test Group Test Group

Anti-inflammation efficacy test

Inflammation model design-Ex vivo 12 weeks Graph
Anti-inflammation related gene expression analysis 4 weeks Graph
B cell- IgE concentration analysis 6 weeks Graph
Mast cell - Histamin or IL-6 analysis 6 weeks Graph
Macrophage - TNFα or IL-6 analysis 6 weeks Graph
Keratinocyte - TARC analysis 6 weeks Graph

Microdust efficacy test

Inflammation or skin barrier related gene expression analysis 8 weeks Graph
Anti-inflammation related gene expression analysis 8 weeks Graph
ROS analysis 8 weeks Image

Skin absorption efficacy test

Absorption analysis in skin model 6 weeks
Images View
Negative Control Group Negative Control Group
Test Group Test Group

Alternatives to the eye irritation test

Retinal cell viability analysis 4 weeks Graph

Anti-hair loss efficacy test

Human follicle dermal papilla cell proliferation analysis 3 weeks Graph
Hair loss related gene expression analysis 4 weeks Graph

Hair improvement efficay test

InfIltration analysis of fluorescent protein to hair 2 weeks/4 weeks Image
Hair roughness analysis 4 weeks
Images View
Control Group Control Group
Test Group Test Group

Anti-microbial activity in vitro

Escherichia coli/Staphylococcus aureus/Candida albicans 3 weeks Image

Anti-microbial efficacy test

Escherichia coli/Staphylococcus aureus/Candida albicans 3 weeks Image

Cytotoxicity test

Cytotoxicity test

Content

Cytotoxicity analysis

Period

3 weeks

Figure/Image

Graph
Test Content Period Figure/Image

Cytotoxicity test

Cytotoxicity analysis 3 weeks Graph

Anti-wrinkle efficacy test

Anti-wrinkle efficacy test

Content

Skin wrinkle related gene expression analysis

Period

4 weeks

Figure/Image

Graph
Anti-wrinkle efficacy test

Content

Collagen1 protein expression analysis

Period

4 weeks

Figure/Image

Graph
Anti-wrinkle efficacy test

Content

Procollagen protein expression analysis

Period

4 weeks

Figure/Image

Graph
Anti-wrinkle efficacy test

Content

MMP1 (collagenase) protein expression analysis

Period

4 weeks

Figure/Image

Graph
Anti-wrinkle efficacy test

Content

Elastase inhibition analysis

Period

4 weeks

Figure/Image

Graph
Anti-wrinkle efficacy test

Content

Aging model design by light (UV or bluelight)-Ex vivo

Period

4 weeks

Figure/Image

Negative Control Group Negative Control Group
UVB-irradiated Group UVB-irradiated Group
Test Group Test Group
Test Content Period Figure/Image

Anti-wrinkle efficacy test

Skin wrinkle related gene expression analysis 4 weeks Graph
Collagen1 protein expression analysis 4 weeks Graph
Procollagen protein expression analysis 4 weeks Graph
MMP1 (collagenase) protein expression analysis 4 weeks Graph
Elastase inhibition analysis 4 weeks Graph
Aging model design by light (UV or bluelight)-Ex vivo 6 weeks
Images View
Negative Control Group Negative Control Group
UVB-irradiated Group UVB-irradiated Group
Test Group Test Group

Whitening efficacy test

Whitening efficacy test

Content

Skin whitening related gene expression analysis

Period

4 weeks

Figure/Image

Graph
Whitening efficacy test

Content

Melanin production analysis

Period

4 weeks

Figure/Image

Graph
Whitening efficacy test

Content

L-DOPA oxidation inhibition analysis

Period

4 weeks

Figure/Image

Graph
Whitening efficacy test

Content

Tyrosinase inhibition analysis

Period

4 weeks

Figure/Image

Graph
Test Content Period Figure/Image

Whitening efficacy test

Skin whitening related gene expression analysis 4 weeks Graph
Melanin production analysis 4 weeks Graph
L-DOPA oxidation inhibition analysis 4 weeks Graph
Tyrosinase inhibition analysis 4 weeks Graph

Anti-oxidant efficay test

Anti-oxidant efficay test

Content

DPPH radical scavenging activity analysis

Period

2 weeks

Figure/Image

Graph
Anti-oxidant efficay test

Content

ORAC antioxidant value analysis

Period

2 weeks

Figure/Image

Graph
Anti-oxidant efficay test

Content

ROS analysis

Period

4 weeks

Figure/Image

Graph
Anti-oxidant efficay test

Content

Antioxidant enzyme analysis in skin corneous

Period

6 weeks

Figure/Image

Graph
Test Content Period Figure/Image

Anti-oxidant efficay test

DPPH radical scavenging activity analysis 2 weeks Graph
ORAC antioxidant value analysis 2 weeks Graph
ROS analysis 4 weeks Graph
Antioxidant enzyme analysis in skin corneous 6 weeks Graph

Anti-diabetic efficacy test

Anti-diabetic efficacy test

Content

α-glucosidase inhibition analysis

Period

4 weeks

Figure/Image

Graph
Test Content Period Figure/Image

Anti-diabetic efficacy test

α-glucosidase inhibition analysis 4 weeks Graph

Anti-glycosylation efficacty test

Anti-glycosylation efficacty test

Content

Advanced glycation end products (AGEs) analysis

Period

4 weeks

Figure/Image

Graph
Anti-glycosylation efficacty test

Content

Denatured collagen production analysis

Period

4 weeks

Figure/Image

Image
Test Content Period Figure/Image

Anti-glycosylation efficacty test

Advanced glycation end products (AGEs) analysis 4 weeks Graph
Denatured collagen production analysis 4 weeks Image

Anti-obesity efficacy test

Anti-obesity efficacy test

Content

Adipogenesis related gene expression analysis

Period

8 weeks

Figure/Image

Graph
Anti-obesity efficacy test

Content

Adipocytes lipid uptake analysis

Period

8 weeks

Figure/Image

Negative Control Group Negative Control Group
Adipogenesis-induced Group Adipogenesis-induced Group
Test Group Test Group
Test Content Period Figure/Image

Anti-obesity efficacy test

Adipogenesis related gene expression analysis 8 weeks Graph
Adipocytes lipid uptake analysis 8 weeks
Images View
Negative Control Group Negative Control Group
Adipogenesis-induced Group Adipogenesis-induced Group
Test Group Test Group

Moisturizing efficacy test

Moisturizing efficacy test

Content

Skin moisturizing related gene expression analysis

Period

4 weeks

Figure/Image

Graph
Moisturizing efficacy test

Content

Skin barrier related gene expression analysis

Period

4 weeks

Figure/Image

Graph
Test Content Period Figure/Image

Moisturizing efficacy test

Skin moisturizing related gene expression analysis 4 weeks Graph
Skin barrier related gene expression analysis 4 weeks Graph

Wound healing efficacy test

Wound healing efficacy test

Content

Scratch wound healing assay

Period

4 weeks

Figure/Image

Negative Control Group Negative Control Group
Test Group Test Group
Test Content Period Figure/Image

Wound healing efficacy test

Scratch wound healing assay 4 weeks
Images View
Negative Control Group Negative Control Group
Test Group Test Group

Anti-inflammation efficacy test

Anti-inflammation efficacy test

Content

Inflammation model design-Ex vivo

Period

12 weeks

Figure/Image

Graph
Anti-inflammation efficacy test

Content

Anti-inflammation related gene expression analysis

Period

4 weeks

Figure/Image

Graph
Anti-inflammation efficacy test

Content

B cell- IgE concentration analysis

Period

6 weeks

Figure/Image

Graph
Anti-inflammation efficacy test

Content

Mast cell - Histamin or IL-6 analysis

Period

6 weeks

Figure/Image

Graph
Anti-inflammation efficacy test

Content

Macrophage - TNFα or IL-6 analysis

Period

6 weeks

Figure/Image

Graph
Anti-inflammation efficacy test

Content

Keratinocyte - TARC analysis

Period

6 weeks

Figure/Image

Graph
Test Content Period Figure/Image

Anti-inflammation efficacy test

Inflammation model design-Ex vivo 12 weeks Graph
Anti-inflammation related gene expression analysis 4 weeks Graph
B cell- IgE concentration analysis 6 weeks Graph
Mast cell - Histamin or IL-6 analysis 6 weeks Graph
Macrophage - TNFα or IL-6 analysis 6 weeks Graph
Keratinocyte - TARC analysis 6 weeks Graph

Microdust efficacy test

Microdust efficacy test

Content

Inflammation or skin barrier related gene expression analysis

Period

8 weeks

Figure/Image

Graph
Microdust efficacy test

Content

Anti-inflammation related gene expression analysis

Period

8 weeks

Figure/Image

Graph
Microdust efficacy test

Content

ROS analysis

Period

8 weeks

Figure/Image

Image
Test Content Period Figure/Image

Microdust efficacy test

Inflammation or skin barrier related gene expression analysis 8 weeks Graph
Anti-inflammation related gene expression analysis 8 weeks Graph
ROS analysis 8 weeks Image

Skin absorption efficacy test

Skin absorption efficacy test

Content

Absorption analysis in skin model

Period

6 weeks

Figure/Image

Negative Control Group Negative Control Group
Test Group Test Group
Test Content Period Figure/Image

Skin absorption efficacy test

Absorption analysis in skin model 6 weeks
Images View
Negative Control Group Negative Control Group
Test Group Test Group

Alternatives to the eye irritation test

Alternatives to the eye irritation test

Content

Retinal cell viability analysis

Period

4 weeks

Figure/Image

Graph
Test Content Period Figure/Image

Alternatives to the eye irritation test

Retinal cell viability analysis 4 weeks Graph

Anti-hair loss efficacy test

Anti-hair loss efficacy test

Content

Human follicle dermal papilla cell proliferation analysis

Period

3 weeks

Figure/Image

Graph
Anti-hair loss efficacy test

Content

Hair loss related gene expression analysis

Period

4 weeks

Figure/Image

Graph
Test Content Period Figure/Image

Anti-hair loss efficacy test

Human follicle dermal papilla cell proliferation analysis 3 weeks Graph
Hair loss related gene expression analysis 4 weeks Graph

Hair improvement efficay test

Hair improvement efficay test

Content

InfIltration analysis of fluorescent protein to hair

Period

2 weeks/4 weeks

Figure/Image

Image
Hair improvement efficay test

Content

Hair roughness analysis

Period

4 weeks

Figure/Image

Control Group Control Group
Test Group Test Group
Test Content Period Figure/Image

Hair improvement efficay test

InfIltration analysis of fluorescent protein to hair 2 weeks/4 weeks Image
Hair roughness analysis 4 weeks
Images View
Control Group Control Group
Test Group Test Group

Anti-microbial activity in vitro

Anti-microbial activity in vitro

Content

Escherichia coli/Staphylococcus aureus/Candida albicans

Period

3 weeks

Figure/Image

Image
Test Content Period Figure/Image

Anti-microbial activity in vitro

Escherichia coli/Staphylococcus aureus/Candida albicans 3 weeks Image

Anti-microbial efficacy test

Anti-microbial efficacy test

Content

Escherichia coli/Staphylococcus aureus/Candida albicans

Period

3 weeks

Figure/Image

Image
Test Content Period Figure/Image

Anti-microbial efficacy test

Escherichia coli/Staphylococcus aureus/Candida albicans 3 weeks Image
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